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Gholve, V. M.
- Effect of Different Culture Media, Temperature, Ph, Carbon and Nitrogen Sourceson Mycelial Growth and Sporulation of Alternaria Carthami Causing Alternaria Blight of Safflower
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1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Prabhani (M.S.), IN
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Prabhani (M.S.), IN
Source
International Journal of Plant Protection, Vol 7, No 2 (2014), Pagination: 349-353Abstract
Eight culture media, eight temperature levels, nine pH levels, seven carbon and six nitrogen sources tested exhibited better growth of Alternaria carthami. Results revealed that Potato dextrose agar gave significantly highest growth (90.00 mm), followed by Potato malt agar (84.16 mm) with excellent sporulation. Temperature levels indicated that highest mean mycelial growth (85.66 mm) was recorded at 30°C followed by 25°C (83.83 mm) and 20°C (66.33 mm). However, maximum mean mycelial growth (85.83 mm) was recorded at pH 6.5 with excellent sporulation, followed by at pH 6 (82.00 mm) and pH 7 (70.33 mm) with excellent and good sporulation, respectively. The carbon sources exhibited varied radial mycelial growth and sporulation of the test pathogen. However, highest radial mycelial growth (86.00 mm) and excellent sporulation was recorded on glucose, followed by on maltose (82.83 mm) and starch (80.33 mm) with excellent sporulation. Nitrogen sources resulted highest radial mycelial growth (82.55 mm) and excellent sporulation on potassium nitrate, followed by on peptone (75.83 mm) with good sporulation. Least radial mycelial growth (19.00 mm) was recorded on urea with poor sporulation.Keywords
Alternaria Carthami, Mycelial Growth, Carbon And Nitrogen Sources
- Effect of Different Culture Media, Temperature and PH on Growth and Sporulation of Alternaria carthami
Abstract Views :274 |
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Authors
Affiliations
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, PARBHANI (M.S.), IN
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, PARBHANI (M.S.), IN
Source
International Journal of Plant Sciences, Vol 10, No 2 (2015), Pagination: 163-167Abstract
All eight culture media tested encouraged better growth of Alternaria carthami. However, Potato dextrose agar gave significantly highest growth (90.00 mm). The second and third best culture media found were Potato malt agar (84.16 mm) and Yeast manitol agar (73.33 mm). Rest of the culture media recorded good amount of mycelial growth in the range of 41.66 mm (Yeast extract agar) to 69.16 mm (Malt extract agar). Colony growth was circular, cottony, grayishblack or olivaceous-black coloured with fair to excellent sporulation. The mean colony growth recorded with all the different temperature ranged from 5.66 mm at 5°C to 85.66 mm at 30°C. However, significantly highest mean mycelial growth (85.66 mm) was recorded at 30°C with excellent (++++) sporulation. The second and third best temperature found were 25°C (83.83 mm) and 20°C (66.33 mm). The mean colony growth recorded with all the pH values ranged from 30.50 mm at pH 4.0 to 85.83 mm at pH 6.5. However, significantly maximum mean mycelial growth (85.83 mm) was recorded at pH 6.5 with excellent (++++) sporulation. The second and third best pH values found were pH 6 (82.00 mm) and pH 7 (70.33 mm) with excellent and good sporulation, respectively.Keywords
Culture Media, Temperature, PH, A. carthami, Growth, Sporulation.- In vitro Efficacy of Fungal and Bacterial Antagonists against Fusarium oxysporum f. sp. Ciceri Causing Chickpea Wilt
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Authors
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1 Department of Plant Pathology, College of Agriculture, Parbhani (M.S.), IN
1 Department of Plant Pathology, College of Agriculture, Parbhani (M.S.), IN
Source
International Journal of Agricultural Sciences, Vol 12, No 2 (2016), Pagination: 215-218Abstract
Fusarium oxysporum f. sp. ciceri is one of the most destructive pathogen, causing wilt disease in chickpea and there by inflicting accountable quantitative (48.29%) as well as qualitative losses. All the six fungal and two bacterial bioagents tested in vitro, exhibited significant mycelial growth inhibition of Fusarium oxysporum f. sp. ciceri. However, Trichoderma viride recorded significantly highest mycelial growth inhibition (75.55%), followed by Trichoderma harzianum (73.77%) Trichoderma koningii (71.88%) and Pseudomonas fluorescens (43.77%). Rest of the bioagents tested also caused significant mycelial inhibition of the test pathogen.Keywords
Fusarium Wilt, Fungal Bioagents, Bacterial Bioagents, Chickpea.References
- Andrabi, M., Vaid, A. and Kumar, R.V. (2011). Evaluation of different measures to control wilt causing pathogens in chickpea. J. Pl. Protec. Res., 51(1) : 55-59.
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- Chavan, T.B. (2004). Studies on Fusarium oxysporum f. sp. ciceri (Padwick) Snyder and Hansen causing wilt of chickpea (Cicer arietinum L.). M.Sc. (Ag.) Thesis, Indira Gandhi Agriculture University, Raipur, C.G. (INDIA).
- Dennis, C. and Webster, J. (1971). Antagonistic properties of species groups of Trichoderma: production of volatile and non-volatile antibiotics. Tr. Br. Mycolog. Soc., 57 : 41-48.
- Duke, J.A. (1981). Handbook of legumes of world economic importance. Plenum Press, NEW YORK, U.S.A.
- Haware, M.P. (1990). Fusarium wilt and other important diseases of chickpea in the Mediterranean area. Options Mediterr. Ser. Semin., 9 : 163-166.
- Huisman, J. and Venderpoel, A.F.B. (1994). Aspect of the nutritional quality and use of cool season food legume in animal food. pp. 53-57.
- Hulse, J.A. (1991). Nature composition and utilization of legumes, pp. 11-27.
- Jalali, B.L. and Chand, H. (1992). Diseases of cereals and pulses. (U.S. Singh, A.N. Mukhopadhayay, J. Kumar, and H. S. Chaube, edi.) Prentice Hall, Englewood Cliffs, NY. 1-429-444.
- Kapoor, S., Jaiswal, A. and Shukla, D.N. (2012). Eco-friendly strategies for management of Fusarium wilt of Pisum sativum (L.). African J. Microbiol. Res., 6(48) : 7397-7400.
- Khilare, V.C., Ahmed, R., Chavan, S.S. and Kohire, O.D. (2009). Management of Fusarium oxysporum f. sp. ciceri by different fungicides. Bioinfolet, 6 : 41-43.
- Korde, M.G. (2011). Studies on Fusarium wilt of chickpea caused by Fusarium oxysporum f. sp. ciceri (Padwik) Synder and Hansan.M.Sc. (Ag.) Thesis, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani, M.S. (INDIA).
- Magar, G.S. (2012). Investigation on wilt of chickpea incited by Fusarium oxysporum f. sp. ciceri (Padwick) Snyder and Hansen. M.Sc. (Ag.) Thesis, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani, M.S. (INDIA).
- McRae, W., (1932). Report on Imperial Mycologists Science Agriculture Research Institute, Pusa, pp. 31-78.
- Mehta, A.N., Chauhan, H.L., Makwana, K.V., Gohel, N.M. and Patel, S.J. (2012). Bioefficacy of phytoextract, antagonist and fungicides against Fusarium udum incitant of pigeonpea wilt. J. Pl. Dis. Sci., 5(1) : 56-60.
- Muehlbauer, F.J. and Rajesh, P.N. (2008). Chickpea, a common source of protein and starch in the semi-arid tropics. Book: Genomics of tropical crop plants. Publisher Springer, N.Y. 1 : 171-186.
- Muehlbauer, F.J., Redden, R.J., Nassib, A.M., Robertson, L.D. and Smithson, J.B. (1988). Population improvement in pulse crops: an assessment of methods and techniques. pp. 943-966. In: R.J. Summerfield (edi.), Kluwer Academic Publishers, Dordrecht, The Netherlands.
- Mulik, M.B. (2009). Studies on wilt of chickpea incited by Fusarium oxysporum f. sp. ciceri (Padwick) Synder and Hansan. M.Sc. (Ag.) Thesis, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani, M.S. (INDIA).
- Nene, Y.L. and Reddy, M.V. (1987). Chickpea diseases and their control. Phytopathology, 42 : 499-505.
- Patil, V.B. (2010). Studies on survey and management of chickpea wilt in Marathwada region. Ph.D. (Ag.) Thesis, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani,M.S. (INDIA).
- Prasad, N. and Padwick, G.W. (1939). The genus Fusarium 11. A species of Fusarium as a cause of wilt of gram (C. arietinum L.). Indian J. Agril. Sci., 9 : 371-380.
- Sangle, U.R. and Bambawale, O.M. (2004). New strains of Trichoderma spp. strongly antagonistic against F. oxysporum f. sp. sesami. J. Mycol. Pl. Patholology, 34 : 181-184.
- Sharma, D. and Jodha, N.S. (1984). Pulse production in semiarid region of India Production of Pulses Production Constraints and Opportunities. pp. 241-265.
- Srivastava, M. and Mall, T.P. (2008). Efficacy of bio-agents and organic amendments against Fusarium udum causing wilt of pigeonpea. Annual Pl. Protect. Sci., 16(1) : 203-267.
- Yadav, S., Mane, S.S. and Ghawade, R.S. (2014). Efficacy of herbicides, fungicides and biological control agents against chickpea wilt. Panjabrao Deshmukh Krishi Vidhypeeth Res. J., 36 (1) : 25-28.
- Nutritional and Physiological Studies of Fusarium oxysporum f. sp. Ciceri (Padwick) Snyder and Hansen Causing Wilt of Chickpea
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Authors
Affiliations
1 Department of Plant Pathology, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN
1 Department of Plant Pathology, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN
Source
International Journal of Plant Sciences, Vol 11, No 2 (2016), Pagination: 213-217Abstract
Nutritional and physiological requirements of Fusarium oxysporum f. sp. ciceri were studied using most virulent isolate FOC-2 (Jalna). Effect of different culture media on mycelial growth of Fusarium oxysporum f. sp. ciceri showed significant differences in growth and sporulation. In vitro, culture media studied, Potato dextrose agar (89.66 mm) and Richard agar medium (85.66) produced significantly highest mean mycelial growth and sporulation. The temperature in the range of 25°C to 30°C and pH having range of 6.0 to 7.0 produce significantly highest mean mycelial growth and sporulation of the test pathogen.Keywords
Nutritional, Physiological, Chickpea.References
- Anonymous (2014). Directorate of Economics and Statistics, Department of Agriculture and Co-operation. Agricultural Statistics at a glance. pp. 94-96.
- Awachar, M.K. (2014). Studies on morphological variability of Fusarium oxysporum f. sp. ciceri causing wilt of chickpea. M.Sc. (Ag.) Thesis, MPKV, Rahuri, Ahmednagar, M.S. (INDIA).
- Butler, E.J. (1918). Fungi and diseases of plants. Book published. (M. C. Saxena, K. B. Singh, edi.), CABI Publishing, CAB Int., Wallingford, UK. 233-270.
- Chaung, T.Y. and Su, H.J. (1989). Study of Fusarium oxysporum f. sp. cubense. Memories of college of Agriculture. National Taiwan University, 28(2):19-26.
- Chavan, T.B. (2004). Studies on Fusarium oxysporum f. sp. ciceri (Padwick) Snyder and Hansen causing wilt of chickpea (Cicer arietinum L.). M.Sc. (Ag.) Thesis, Indira Gandhi Agriculture University, Raipur, C.G. (INDIA).
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- Haware, M.P. (1990). Fusarium wilt and other important diseases of chickpea in the Mediterranean area. Options Mediterr. Ser. Semin., 9 : 163-166.
- Jalali, B.L. and Chand, H. (1992). Diseases of cereals and pulses. (U. S. Singh, A. N. Mukhopadhayay, J. Kumar, and H. S. Chaube, edi.) Prentice Hall, Englewood Cliffs, NY. 1-429-444.
- Kadam, N. (2012). Molecular characterization of different isolates of Fusarium oxysporum f. sp. ciceri causing chickpea wilt from Maharashtra. M.Sc. (Ag.) Thesis, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, M.S. (INDIA).
- Khan, H.S., Saifulla M., Mahesh, S.B. and Pallavi, M.S. (2011). Effect of different media and environmental conditions on the growth of Fusarium oxysporum f. sp. ciceri causing Fusarium wilt of chickpea.
- Khilare, V.C. and Ahmed, R. (2012). Effect of different media, pH and temperature on the growth of Fusarium oxysporum f. sp. ciceri causing chickpea wilt. Internat. J. Advan. Bio. Res., 2(1) : 99-102.
- Khilare, V.C., Ahmed, R., Chavan, S.S. and Kohire, O.D. (2009). Management of Fusarium oxysporum f. sp. ciceri by different fungicides. Bioinfolet., 6 : 41-43.
- McRae, W. (1932). Report on Imperial Mycologists Science Agriculture Research Institute, Pusa. pp. 31-78.
- Nene, Y.L. and Reddy, M.V. (1987). Chickpea diseases and their control. Phytopatholgy, 42: 499-505.
- Osman, M., Sayed, M.A., Mohamed, Y.A.H. and Metwally, M. (1992). Effect of various cultural conditions culture media, temperature and carbon source on Alternaria alternata and Fusarium oxysporum. Microbios, 71 (286) : 15-26.
- Prasad, N. and Padwick, G.W. (1939). The genus Fusarium 11. A species of Fusarium as a cause of wilt of gram (C. arietinum L.). Indian J. Agric. Sci., 9 : 371-380.
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- Exploration of Fungicides against Fusarium oxysporum f. sp. Ciceri (Padwick) Snyder and Hansen Causing Wilt of Chickpea
Abstract Views :236 |
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Authors
Affiliations
1 Department of Plant Pathology, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN
1 Department of Plant Pathology, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN
Source
International Journal of Plant Sciences, Vol 11, No 2 (2016), Pagination: 255-261Abstract
Six systemic, six non-systemic and contact fungicides were evaluated, in vitro against Fusarium oxysporum f. sp. ciceri; however, the percentage mycelial growth inhibition was found to be increased with increase in concentrations of the test fungicides. All the systemic, non-systemic and contact fungicides tested, however, among the systemic fungicides, carbendazim, carboxin and benomyl and among non-systemic and contact fungicides, carbendazim + mancozeb, benomyl+ thiram and carbendazim + thiram were found inhibit the growth of the test pathogen completely .Keywords
Systemic Fungicides, Non-Systemic Fungicides, Contact Fungicides.References
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- Anonymous (2014). Directorate of Economics and Statistics, Department of Agriculture and Co-operation. Agricultural statistics at a glance. pp: 94-96.
- Aykoid, W.R. and Doughty, J. (1964). Legume in human nutrition. FAO nutritional studies. 9 pp.
- Butler, E.J. (1918). Fungi and diseases of plants. Book published. (M. C. Saxena, K. B. Singh, edi.), CABI Publishing, CAB Int. Wallingford, UK. 233-270.
- Chavan, T.B. (2004). Studies on Fusarium oxysporum f. sp. ciceri (Padwick) Snyder and Hansen causing wilt of chickpea (Cicer arietinum L.). M.Sc. (Ag.) Thesis, Indira Gandhi Agriculture University, Raipur, C.G. (INDIA).
- Dikkar, M., Jani, S.M. and Deshmukh, V.V. (2001). Effect of fungicides against Fusarium oxysporum f. sp. ciceri, a chickpea wilt pathogen. Ann. Pl. Physiol., 15 (2): 147-149.
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- Muehlbauer, F.J., Redden, R.J., Nassib, A.M., Robertson, L.D. and Smithson, J.B. (1988). Population improvement in pulse crops: an assessment of methods and techniques. pp: 943-966. In: R.J. Summerfield (edi.), Kluwer Academic Publishers, Dordrecht, The Netherlands.
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- Patil, V.B. (2010). Studies on survey and management of chickpea wilt in Marathwada region. Ph.D. (Ag.) Thesis, Vasantrao Naik Marathwara Krishi Vidyapeeth, Parbhani, M.S. (INDIA).
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- Survey for Incidence and Severity of Alternaria Blight of Safflower in Different Districts of Marathwada Region
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1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN
Source
International Journal of Plant Protection, Vol 9, No 1 (2016), Pagination: 219-222Abstract
Survey was conducted in four districts of Marathwada region of Maharashtra during Rabi 2013 to know the incidence and intensity of leaf blight disease caused by Alternaria carthami. The disease was predominant in all the districts surveyed. Highest per cent disease incidence and intensity was reported in Hingoli (42.08 and 38.46 %, respectively). This was followed by Nanded (40.07 and 36.03 %, respectively).Keywords
Alternaria Blight, Incidence, Carthamus tinctorius, Severity Survey.- Isolation, Identification, Pathogenicity Test and Screening of Brinjal Cultivars against Damping off in Brinjal
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Authors
Affiliations
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN